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pituitary adenylate cyclase activating peptide 1 27  (Tocris)
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pacap  (MedChemExpress)
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pacap 1 27  (Tocris)
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pacap 1 27  (MedChemExpress)
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125 i-ac-his 1 ]pacap-1-27  (ANAWA Inc)
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rabbit polyclonal antibodies against pacap 1-38 pacap 1-27  (Euro Diagnostica)
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Double-immunofluorescence staining for <t>PACAP</t> <t>(polyclonal,</t> green) and mast cell tryptase (monoclonal, red) in tissues from patients with urticaria (n = 6). A: Marked immunostaining for PACAP (green) in nerve fibers close to the dermal-epidermal border of human skin tissue (arrows). In the environment of epidermal nerve fibers, single PACAP-positive dendritic-like cells (Langerhans cells) of urticaria patients were detected (arrowhead, red; ×20; Scale bar = 40 μm). B: In the upper dermis, marked immunofluorescence staining for PACAP in nerve fibers (arrows) closely accompanied by tryptase-positive mast cells (red; ×40; Scale bar = 31.5 μm). C: PACAP-positive nerve fibers (arrows) close to dermal blood vessels (arrowhead, ×40; Scale bar = 31.5 μm). D: Negative control (pre-immune absorption control) showing absence of PACAP in nerve fibers and endothelial cells demonstrating specificity of immunostaining (×10; Scale bar = 45 μm).
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pacap(1–27) peptide  (GL Biochem)
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Double-immunofluorescence staining for <t>PACAP</t> <t>(polyclonal,</t> green) and mast cell tryptase (monoclonal, red) in tissues from patients with urticaria (n = 6). A: Marked immunostaining for PACAP (green) in nerve fibers close to the dermal-epidermal border of human skin tissue (arrows). In the environment of epidermal nerve fibers, single PACAP-positive dendritic-like cells (Langerhans cells) of urticaria patients were detected (arrowhead, red; ×20; Scale bar = 40 μm). B: In the upper dermis, marked immunofluorescence staining for PACAP in nerve fibers (arrows) closely accompanied by tryptase-positive mast cells (red; ×40; Scale bar = 31.5 μm). C: PACAP-positive nerve fibers (arrows) close to dermal blood vessels (arrowhead, ×40; Scale bar = 31.5 μm). D: Negative control (pre-immune absorption control) showing absence of PACAP in nerve fibers and endothelial cells demonstrating specificity of immunostaining (×10; Scale bar = 45 μm).
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ac-[phe(pi)(6), nle(17)]pacap(1-27)  (Neuropharm Ltd)
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Double-immunofluorescence staining for <t>PACAP</t> <t>(polyclonal,</t> green) and mast cell tryptase (monoclonal, red) in tissues from patients with urticaria (n = 6). A: Marked immunostaining for PACAP (green) in nerve fibers close to the dermal-epidermal border of human skin tissue (arrows). In the environment of epidermal nerve fibers, single PACAP-positive dendritic-like cells (Langerhans cells) of urticaria patients were detected (arrowhead, red; ×20; Scale bar = 40 μm). B: In the upper dermis, marked immunofluorescence staining for PACAP in nerve fibers (arrows) closely accompanied by tryptase-positive mast cells (red; ×40; Scale bar = 31.5 μm). C: PACAP-positive nerve fibers (arrows) close to dermal blood vessels (arrowhead, ×40; Scale bar = 31.5 μm). D: Negative control (pre-immune absorption control) showing absence of PACAP in nerve fibers and endothelial cells demonstrating specificity of immunostaining (×10; Scale bar = 45 μm).
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pacap (1-27) ria  (Peninsula Laboratories)
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Double-immunofluorescence staining for <t>PACAP</t> <t>(polyclonal,</t> green) and mast cell tryptase (monoclonal, red) in tissues from patients with urticaria (n = 6). A: Marked immunostaining for PACAP (green) in nerve fibers close to the dermal-epidermal border of human skin tissue (arrows). In the environment of epidermal nerve fibers, single PACAP-positive dendritic-like cells (Langerhans cells) of urticaria patients were detected (arrowhead, red; ×20; Scale bar = 40 μm). B: In the upper dermis, marked immunofluorescence staining for PACAP in nerve fibers (arrows) closely accompanied by tryptase-positive mast cells (red; ×40; Scale bar = 31.5 μm). C: PACAP-positive nerve fibers (arrows) close to dermal blood vessels (arrowhead, ×40; Scale bar = 31.5 μm). D: Negative control (pre-immune absorption control) showing absence of PACAP in nerve fibers and endothelial cells demonstrating specificity of immunostaining (×10; Scale bar = 45 μm).
Pacap (1 27) Ria, supplied by Peninsula Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pacap-1-27  (Bachem)
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Double-immunofluorescence staining for <t>PACAP</t> <t>(polyclonal,</t> green) and mast cell tryptase (monoclonal, red) in tissues from patients with urticaria (n = 6). A: Marked immunostaining for PACAP (green) in nerve fibers close to the dermal-epidermal border of human skin tissue (arrows). In the environment of epidermal nerve fibers, single PACAP-positive dendritic-like cells (Langerhans cells) of urticaria patients were detected (arrowhead, red; ×20; Scale bar = 40 μm). B: In the upper dermis, marked immunofluorescence staining for PACAP in nerve fibers (arrows) closely accompanied by tryptase-positive mast cells (red; ×40; Scale bar = 31.5 μm). C: PACAP-positive nerve fibers (arrows) close to dermal blood vessels (arrowhead, ×40; Scale bar = 31.5 μm). D: Negative control (pre-immune absorption control) showing absence of PACAP in nerve fibers and endothelial cells demonstrating specificity of immunostaining (×10; Scale bar = 45 μm).
Pacap 1 27, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pacap-(1–27)  (Bachem)
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Bachem pacap-(1–27)
Double-immunofluorescence staining for <t>PACAP</t> <t>(polyclonal,</t> green) and mast cell tryptase (monoclonal, red) in tissues from patients with urticaria (n = 6). A: Marked immunostaining for PACAP (green) in nerve fibers close to the dermal-epidermal border of human skin tissue (arrows). In the environment of epidermal nerve fibers, single PACAP-positive dendritic-like cells (Langerhans cells) of urticaria patients were detected (arrowhead, red; ×20; Scale bar = 40 μm). B: In the upper dermis, marked immunofluorescence staining for PACAP in nerve fibers (arrows) closely accompanied by tryptase-positive mast cells (red; ×40; Scale bar = 31.5 μm). C: PACAP-positive nerve fibers (arrows) close to dermal blood vessels (arrowhead, ×40; Scale bar = 31.5 μm). D: Negative control (pre-immune absorption control) showing absence of PACAP in nerve fibers and endothelial cells demonstrating specificity of immunostaining (×10; Scale bar = 45 μm).
Pacap (1–27), supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pacap 1–27  (Peninsula Laboratories)
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Peninsula Laboratories pacap 1–27
Double-immunofluorescence staining for <t>PACAP</t> <t>(polyclonal,</t> green) and mast cell tryptase (monoclonal, red) in tissues from patients with urticaria (n = 6). A: Marked immunostaining for PACAP (green) in nerve fibers close to the dermal-epidermal border of human skin tissue (arrows). In the environment of epidermal nerve fibers, single PACAP-positive dendritic-like cells (Langerhans cells) of urticaria patients were detected (arrowhead, red; ×20; Scale bar = 40 μm). B: In the upper dermis, marked immunofluorescence staining for PACAP in nerve fibers (arrows) closely accompanied by tryptase-positive mast cells (red; ×40; Scale bar = 31.5 μm). C: PACAP-positive nerve fibers (arrows) close to dermal blood vessels (arrowhead, ×40; Scale bar = 31.5 μm). D: Negative control (pre-immune absorption control) showing absence of PACAP in nerve fibers and endothelial cells demonstrating specificity of immunostaining (×10; Scale bar = 45 μm).
Pacap 1–27, supplied by Peninsula Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Double-immunofluorescence staining for PACAP (polyclonal, green) and mast cell tryptase (monoclonal, red) in tissues from patients with urticaria (n = 6). A: Marked immunostaining for PACAP (green) in nerve fibers close to the dermal-epidermal border of human skin tissue (arrows). In the environment of epidermal nerve fibers, single PACAP-positive dendritic-like cells (Langerhans cells) of urticaria patients were detected (arrowhead, red; ×20; Scale bar = 40 μm). B: In the upper dermis, marked immunofluorescence staining for PACAP in nerve fibers (arrows) closely accompanied by tryptase-positive mast cells (red; ×40; Scale bar = 31.5 μm). C: PACAP-positive nerve fibers (arrows) close to dermal blood vessels (arrowhead, ×40; Scale bar = 31.5 μm). D: Negative control (pre-immune absorption control) showing absence of PACAP in nerve fibers and endothelial cells demonstrating specificity of immunostaining (×10; Scale bar = 45 μm).

Journal:

Article Title: Pituitary Adenylate Cyclase Activating Polypeptide

doi: 10.2353/ajpath.2010.090941

Figure Lengend Snippet: Double-immunofluorescence staining for PACAP (polyclonal, green) and mast cell tryptase (monoclonal, red) in tissues from patients with urticaria (n = 6). A: Marked immunostaining for PACAP (green) in nerve fibers close to the dermal-epidermal border of human skin tissue (arrows). In the environment of epidermal nerve fibers, single PACAP-positive dendritic-like cells (Langerhans cells) of urticaria patients were detected (arrowhead, red; ×20; Scale bar = 40 μm). B: In the upper dermis, marked immunofluorescence staining for PACAP in nerve fibers (arrows) closely accompanied by tryptase-positive mast cells (red; ×40; Scale bar = 31.5 μm). C: PACAP-positive nerve fibers (arrows) close to dermal blood vessels (arrowhead, ×40; Scale bar = 31.5 μm). D: Negative control (pre-immune absorption control) showing absence of PACAP in nerve fibers and endothelial cells demonstrating specificity of immunostaining (×10; Scale bar = 45 μm).

Article Snippet: Frozen sections (10–14 μm) were preincubated for 30 minutes with normal serum and incubated overnight at 4°C with mouse monoclonal antibodies against PACAP 1-38 (1:10; kindly provided by Dr. J. Fahrenkrug, Department of Clinical Biochemistry, Copenhagen, Denmark); mouse monoclonal antibodies against PACAP 1-27 or PACAP 1-38 (1:200; 37 ), or rabbit polyclonal antibodies against PACAP 1-38 and PACAP 1-27 (1:1000, Euro Diagnostica, Malmo, Sweden).

Techniques: Double Immunofluorescence Staining, Immunostaining, Immunofluorescence, Staining, Negative Control